Evaluation of Chloroform Extract and Its Fractions of Ocimum gratissimum (Linn.) Leaves for Cardioprotective Activity

Abstract

The present study has been designed to investigate the efficacy of active extract and its fraction of Ocimum gratissimum (Linn.) leaves on ischaemia and reperfusion-induced myocardial injury using wister albino rats. Petroleum ether, chloroform, acetone and methanolic extracts of Ocimum gratissimum (Linn.) leaves were prepared and screened for their cardioprotective potential. Among all the extracts chloroform extract reduce myocardial injury. The chloroform extract further purified using column chromatographic technique that results four major fractions viz F1, F2, F3 and F4. These fractions screened for myocardial infarct size and LDH and CK-MB release in coronary effluent at dose level of 100mg/kg body weight as compared with that of standard Trimetazidine(35mg/kg body weight). The fraction F4 of chloroform extract significantly prevented myocardial infarct size and LDH and CK-MB release as compared with that of standard trimetazidine. Further, cardioprotective effect of the fraction F4 of chloroform extracts of Ocimum gratissimum (Linn.) was significantly (pE‚0.05) compared to vehicle control group.