Phylogenetic study of Puntius cf. binotatus fish from Gunung Tujuh Lake in Sumatera Based on Cytochrome b Gene

Abstract

Research on the study ofrnphylogenetic Puntius cf. binotatus from Gunung Tujuh Lake basedrnon cytochrome b gene. The study aimed to determine the phylogeneticrnrelationships between Puntius cf. binotatus from Gunung TujuhrnLake and Puntius binotatus from other populations in West Sumatra. DNA sequences were alignment using ClustalrnX version 1.8 and phylogenetic tree was analyzed using MEGA 6.0. The alignment showed thernnumber of partial of the cytochrome b gene was 530 base pair. The results of the study showed that the geneticrndivergences between them were 6.6%-11.2%. Therefore, it was possible to place the Puntius cf. binotatus as new speciesrnwith its two unique haplotypes and from the tree,rnwe concluded that there are at least four subspecies or species from ourrnsamples