Response surface methodology optimization of demineralization from sardine (Sardina pilchardus) scale

Abstract

Sardine scales can servernas an additional source of proteins, especially of collagen. To obtain nativerncollagen previous deproteinization and demineralization of scales arernnecessary. Therefore, the aim of this work is to determine optimal parametersrnfor demineralization of sardine scales by complexing metal ions with ethylenediaminetetraaceticrnacid (EDTA). In this study, the response surface methodology is used to build arnmodel to predict and optimize the demineralization process. The optimalrnconditions are X1=0,3M (EDTA concentration) X2=18h (EDTA treatment time). Underrnthis optimal condition, the experimental values agreed with the predictedrnvalues, using analysis of variance, indicating a high goodness of fit of thernmodel used and the success of response surface methodology for modelingrndemineralization extraction of scale sardine