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Improved procedure for the extraction of lipids from human erythrocytes
Journal of Lipid Research · 1965 · Vol. 6(3) · pp. 428–431
Abstract
A procedure for the extraction of human erythrocyte lipids using chloroform–isopropanol 7:11 (v/v) is described. It is simple and reproducible, affords almost quantitative extraction of cholesterol and phospholipid, uses a single extraction tube, and yields an extract only slightly contaminated by heme.
Mass Spectrometry Techniques and ApplicationsMetabolomics and Mass Spectrometry StudiesErythrocyte Function and PathophysiologyExtraction (chemistry)ChromatographyChloroformChemistryPhospholipidHemeBiochemistryMembraneEnzyme
MeSH terms
ErythrocytesLipids
Citations
1,047
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4.93
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13
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97%
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References
Microdetermination of Long-chain Fatty Acids in Plasma and Tissues
Journal of Biological Chemistry · 1960 · 2,219 citations
The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes
Archives of Biochemistry and Biophysics · 1963 · 4,410 citations
A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Journal of Biological Chemistry · 1957 · 64,580 citations
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