In vitro Anti-Oxidant, Anti-Nociceptive and Anti-Inflammatory Properties of Pongamia Pinnata Stem Bark in Experimental Animal Models

Abstract

This study aims to evaluate the anti-inflammatory and anti-nociceptivepotential of the ethanolic extract of pongamia pinnata stem bark(PSBE) in different experimental animal models.The antioxidant activity of ethanolic extract ofpongamia pinnataand compared with ascorbic acid (Standard) and theanalgesic and anti-inflammatory activities in animal models. The extract has an anti-inflammatory effect demonstrated by its inhibitory effects on Carrageenan induced paw edema.PSBE (200, 500 and 1000 mg/kg) exhibited significant anti-inflammatory activity in acute (carrageenan induced hind paw edema) and chronic (cotton pellet granuloma) models of inflammationPSBE did not show any sign of toxicity and mortality up to a dose level of 10.125 g/kg, p.o. in mice. Both acute as well as chronic administration PSBE (200, 500 and 1000 mg/kg, p.o.) did not produce any gastric lesion in rats.The analgesic activity was tested by acetic acid-induced writhing response in albino mice and tail flick method in albino rats.Its ethanolic extract shows the most effective anti-inflammatory activityat doses of 200, 500 and 1000 mg/kg significantly throughout the observation period.In the tail flick model, the PSBE in the above doses increased the pain threshold significantly after 30 min., 1, 2, and 4 hr. of administration. pongamia pinnatashowed dose-dependent action in all experimental animal models.