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Development and implementation of high-throughput SNP genotyping in barley

BMC Genomics · 2009 · Vol. 10(1) · pp. 582–582

Timothy J. Close✉(University of California, Riverside)Prasanna R. Bhat(Monsanto (United States))Stefano Lonardi(University of California, Riverside)Yonghui Wu(Google (United States))Nils Rostoks(University of Latvia)Luke RamsayArnis DrukaNils Stein(Leibniz Institute of Plant Genetics and Crop Plant Research)Jan T. Svensson(Frederiksberg Hospital)Steve Wanamaker(University of California, Riverside)Serdar Bozdag(National Cancer Institute)Mikeal L. Roose(University of California, Riverside)Matthew Moscou(Iowa State University)Shiaoman Chao(Edward T. Schafer Agricultural Research Center)Rajeev K. Varshney(International Crops Research Institute for the Semi-Arid Tropics)Péter Szűcs(Oregon State University)Kazuhiro Sato(Okayama University)Patrick M. Hayes(Oregon State University)David E. Matthews(Cornell University)Andris Kleinhofs(Washington State University)Gary J. Muehlbauer(University of Minnesota)Joseph DeYoung(Southern California Reproductive Center)David MarshallKavitha Madishetty(University of California, Riverside)Raymond D. Fenton(University of California, Riverside)Pascal Condamine(University of California, Riverside)Andreas Graner(Leibniz Institute of Plant Genetics and Crop Plant Research)Robbie Waugh

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Abstract

The unprecedented density of genic markers and marker bins enabled a high resolution comparison of the genomes of barley and rice. Low recombination in pericentric regions is evident from bins containing many more than the average number of markers, meaning that a large number of genes are recombinationally locked into the genetic centromeric regions of several barley chromosomes. Examination of US breeding germplasm illustrated the usefulness of BOPA1 and BOPA2 in that they provide excellent marker density and sensitivity for detection of minor alleles in this genetically narrow material.

Wheat and Barley Genetics and Pathology Plant tissue culture and regeneration Genetic Mapping and Diversity in Plants and AnimalsBiologyGeneticsTag SNPSNP genotypingSNPGenotypingSingle-nucleotide polymorphismGermplasmGene mappingPopulation