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Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction

Analytical Biochemistry · 1997 · Vol. 245(2) · pp. 154–160
Kirk M. RirieRandy RasmussenCarl T. Wittwer
Animal Genetics and ReproductionMolecular Biology Techniques and ApplicationsAdvanced biosensing and bioanalysis techniquesMelting curve analysisSYBR Green IFluorometerChemistryMelting temperaturePrimer dimerDNAFluorescencePolymerase chain reactionNucleic acid thermodynamics

MeSH terms

DiaminesDNAElectrophoresisFluorescenceFluorescent DyesFluorometryOrganic ChemicalsQuinolinesTemperaturePolymerase Chain ReactionBenzothiazoles
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