articleTop 1% cited

Digital holographic microscopy for live cell applications and technical inspection

Applied Optics · 2007 · Vol. 47(4) · pp. A52–A52

Björn Kemper✉(University of Münster)Gert von Bally(University of Münster)

Abstract

Digital holographic microscopy enables a quantitative phase contrast metrology that is suitable for the investigation of reflective surfaces as well as for the marker-free analysis of living cells. The digital holographic feature of (subsequent) numerical focus adjustment makes possible applications for multifocus imaging. An overview of digital holographic microscopy methods is described. Applications of digital holographic microscopy are demonstrated by results obtained from livings cells and engineered surfaces.

Digital Holography and Microscopy Advanced Optical Imaging Technologies Image Processing Techniques and ApplicationsDigital holographic microscopyHolographyDigital holographyMicroscopyOpticsPhase contrast microscopyHolographic interferometryMetrologyMaterials scienceFocus (optics)

MeSH terms

AlgorithmsEquipment DesignHolographyHumansImage EnhancementImage Interpretation, Computer-AssistedMaterials TestingMicroscopy, Phase-ContrastPancreatic NeoplasmsSensitivity and SpecificitySignal Processing, Computer-AssistedReproducibility of ResultsEquipment Failure AnalysisCell Line, Tumor

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References

Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms