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Resazurin Microtiter Assay Plate: Simple and Inexpensive Method for Detection of Drug Resistance in Mycobacterium tuberculosis

Antimicrobial Agents and Chemotherapy · 2002 · Vol. 46(8) · pp. 2720–2722

Juan-Carlos Palomino✉(Instituut voor Tropische Geneeskunde)Anandi Martin(Instituut voor Tropische Geneeskunde)Mirtha CamachoHumberto Guerra(Universidad Peruana Cayetano Heredia)Jean Swings(Ghent University)Françoise Portaels(Instituut voor Tropische Geneeskunde)

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Abstract

A method for detecting multidrug-resistant Mycobacterium tuberculosis by using a reduction of resazurin is described. Eighty clinical isolates were evaluated against isoniazid and rifampin; results at 7 days were compared with those of the proportion method. Specificity and sensitivity were excellent. The method is simple, inexpensive, and rapid and might be used with other antituberculosis drugs.

Tuberculosis Research and Epidemiology Mycobacterium research and diagnosis Infectious Diseases and TuberculosisResazurinMicrotiter plateMycobacterium tuberculosisDrug resistanceMicrobiologyTuberculosisMycobacteriumBiologyChromatographyVirology

MeSH terms

Antitubercular AgentsColorimetryDrug Resistance, MicrobialHumansIndicators and ReagentsIsoniazidMicrobial Sensitivity TestsMycobacterium tuberculosisOxazinesPeruRifampinTuberculosisXanthenes

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Thiosemicarbazones, semicarbazones, dithiocarbazates and hydrazide/hydrazones: Anti – Mycobacterium tuberculosis activity and cytotoxicity