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LOCALIZATION OF ANTIGEN IN TISSUE CELLS
The Journal of Experimental Medicine · 1950 · Vol. 91(1) · pp. 1–13
Albert H. Coons✉(Harvard University)Melvin H. Kaplan(Harvard University)
Abstract
Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two isomeric series derived from nitrofluorescein are described.
Monoclonal and Polyclonal Antibodies ResearchAntigenFluoresceinStainAntibodyFluorescence microscopeFluorescenceMolecular biologyStainingBiologyChemistry
MeSH terms
AntibodiesAntigensFluorescent Antibody TechniqueHumansImmunoglobulins
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