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Fading of immunofluorescence during microscopy: a study of the phenomenon and its remedy

Journal of Immunological Methods · 1982 · Vol. 55(2) · pp. 231–242
G.D. JohnsonR. Stephen DavidsonK.C. McNameeG. I. RussellDean GoodwinE. J. Holborow
Advanced Biosensing Techniques and ApplicationsPhotodynamic Therapy Research StudiesMonoclonal and Polyclonal Antibodies ResearchFadingSinglet oxygenChemistryFluorescenceFluorescence microscopeMicroscopyImmunofluorescenceOctaneKineticsGlycerol

MeSH terms

AzidesFluoresceinsFluorescent Antibody TechniqueGlycerolHumansMicroscopy, FluorescencePhenylenediaminesPiperazinesSodium IodideThiocyanatesFluorescein-5-isothiocyanateSodium Azide
Citations
812
FWCI
11.15
field-weighted impact
References
17
Percentile
99%
vs. same field & year
Citations per year
References
A simple method of reducing the fading of immunofluorescence during microscopy
Journal of Immunological Methods · 1981 · 1,885 citations
LOCALIZATION OF ANTIGEN IN TISSUE CELLS
The Journal of Experimental Medicine · 1950 · 1,904 citations
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