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Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

Genetics · 2010 · Vol. 186(2) · pp. 757–761

Michelle Christian(University of Minnesota)Tomáš Čermák(University of Minnesota)Erin Doyle(Iowa State University)Clarice Schmidt(Iowa State University)Feng Zhang(University of Minnesota)Aaron W. Hummel(Iowa State University)Adam J. Bogdanove(Iowa State University)Daniel F. Voytas✉(University of Minnesota)

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Abstract

Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.

CRISPR and Genetic Engineering RNA Interference and Gene Delivery Advanced biosensing and bioanalysis techniquesTranscription activator-like effector nucleaseBiologyNucleaseGenome editingEffectorDNACleaveGeneticsEndonucleaseMutagenesis

MeSH terms

Binding SitesDNADNA RepairDNA-Binding ProteinsGenetic EngineeringRecombinant Fusion ProteinsTranscription FactorsTranscription, GeneticXanthomonasDeoxyribonucleases, Type II Site-SpecificTranscriptional ActivationZinc FingersXanthomonas campestrisGene TargetingCatalytic Domain