articleTop 10% cited
Construction and analysis of<i>luxCDABE</i>-based plasmid sensors for investigating<i>N</i>-acyl homoserine lactone-mediated quorum sensing
FEMS Microbiology Letters · 1998 · Vol. 163(2) · pp. 185–192
Michael K. Winson(University of Nottingham)Simon Swift✉(University of Nottingham)Leigh Fish(University of Nottingham)John Throup(University of Nottingham)Frieda JÃ ̧rgensen(University of Nottingham)Siri Ram Chhabra(University of Nottingham)Barrie W. Bycroft(University of Nottingham)Paul Williams(University of Nottingham)Gordon S.A.B. Stewart(University of Nottingham)
Abstract
Plasmid reporter vectors have been constructed which respond to activation of LuxR and its homologues LasR and RhlR (VsmR) by N-acyl homoserine lactones (AHLs). The expression of luxCDABE from transcriptional fusions to PluxI, PlasI and PrhlI respectively, occurs in the presence of activating AHLs. A profile of structure/activity relationships is seen where the natural ligand is most potent. The characterisation of individual LuxR homologue/AHL combinations allows a comprehensive evaluation of quorum sensing signals from a test organism.
Bacterial biofilms and quorum sensingAntimicrobial Peptides and ActivitiesBacterial Genetics and BiotechnologyQuorum sensingBioreporterHomoserinePlasmidReporter geneBiologyGeneMicrobiologyBiochemistryGene expression
MeSH terms
Bacterial ProteinsConjugation, GeneticDNA-Binding ProteinsEscherichia coliGenetic VectorsLuminescent MeasurementsPlasmidsRepressor ProteinsStructure-Activity Relationship4-ButyrolactoneSignal TransductionTrans-ActivatorsGene Expression Regulation, BacterialGenes, Reporter
Funding
- European Commission
Citations
586
FWCI
5.28
field-weighted impact
References
20
Percentile
96%
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References
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Gene · 1985 · 15,158 citations
Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa
Journal of Bacteriology · 1997 · 901 citations
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Construction and analysis of<i>luxCDABE</i>-based plasmid sensors for investigating<i>N</i>-acyl homoserine lactone-mediated quorum sensing
FEMS Microbiology Letters · 1998 · 586 citations
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