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Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions
Nature Protocols · 2012 · Vol. 8(1) · pp. 162–175
Xiaojun Lian✉(University of Wisconsin–Madison)Jianhua Zhang(University of Wisconsin–Madison)Samira M. Azarin(University of Wisconsin–Madison)Kexian Zhu(University of Wisconsin–Madison)Laurie B. Hazeltine(University of Wisconsin–Madison)Xiaoping Bao(University of Wisconsin–Madison)Cheston Hsiao(University of Wisconsin–Madison)Timothy J. Kamp(University of Wisconsin–Madison)Sean P. Palecek(University of Wisconsin–Madison)
Pluripotent Stem Cells ResearchTissue Engineering and Regenerative MedicineCongenital heart defects researchWnt signaling pathwayInduced pluripotent stem cellCell biologyDirected differentiationBiologyStem cellCellular differentiationCell sortingFlow cytometrySignal transduction
MeSH terms
Cell DifferentiationFlow CytometryHumansLentivirusCell Culture TechniquesMyocytes, CardiacRNA, Small InterferingPluripotent Stem Cellsbeta CateninWnt Signaling Pathway
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References
Stage-Specific Optimization of Activin/Nodal and BMP Signaling Promotes Cardiac Differentiation of Mouse and Human Pluripotent Stem Cell Lines
Cell stem cell · 2011 · 1,171 citations
<i>Nkx-2</i>.<i>5</i>: a novel murine homeobox gene expressed in early heart progenitor cells and their myogenic descendants
Development · 1993 · 865 citations
Functional Cardiomyocytes Derived From Human Induced Pluripotent Stem Cells
Circulation Research · 2009 · 1,400 citations
Cardiomyocytes derived from human embryonic stem cells in pro-survival factors enhance function of infarcted rat hearts
Nature Biotechnology · 2007 · 2,204 citations
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