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Differentiation of the Major <i>Listeria monocytogenes</i> Serovars by Multiplex PCR

Journal of Clinical Microbiology · 2004 · Vol. 42(8) · pp. 3819–3822

Michel Doumith✉(World Health Organization)Carmen Buchrieser(Institut Pasteur)Philippe Glaser(Institut Pasteur)Christine Jacquet(World Health Organization)Paul Martin(World Health Organization)

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Abstract

A new multiplex PCR assay was developed to separate the four major Listeria monocytogenes serovars isolated from food and patients (1/2a, 1/2b, 1/2c, and 4b) into distinct groups. The PCR test, which constitutes a rapid and practical alternative to laborious classical serotyping, was successfully evaluated with 222 Listeria strains.

Listeria monocytogenes in Food Safety Salmonella and Campylobacter epidemiology Identification and Quantification in FoodListeria monocytogenesSerotypeMultiplex polymerase chain reactionListeriaMicrobiologyBiologyMultiplexPolymerase chain reactionFood microbiologyVirology

MeSH terms

Base SequenceDNA, BacterialElectrophoresis, Agar GelFood MicrobiologyHumansListeriaListeriosisListeria monocytogenesSerotypingReproducibility of ResultsPolymerase Chain ReactionDNA Primers

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A New Perspective on Listeria monocytogenes Evolution