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Applications of random amplified polymorphic DNA (RAPD) in molecular ecology

Molecular Ecology · 1992 · Vol. 1(1) · pp. 55–63

Heike Hadrys✉(Yale University)Michael J. Balick(New York Botanical Garden)Bernd Schierwater(New York Botanical Garden)

Abstract

Molecular genetic markers have been developed into powerful tools to analyse genetic relationships and genetic diversity. As an extension to the variety of existing techniques using polymorphic DNA markers, the Random Amplified Polymorphic DNA (RAPD) technique may be used in molecular ecology to determine taxonomic identity, assess kinship relationships, analyse mixed genome samples, and create specific probes. Main advantages of the RAPD technology include (i) suitability for work on anonymous genomes, (ii) applicability to problems where only limited quantities of DNA are available, (iii) efficiency and low expense.

Environmental DNA in Biodiversity Studies Genetic diversity and population structure Identification and Quantification in FoodRAPDBiologyMolecular ecologyGenomeGenetic diversityEvolutionary biologyDNAGeneticsGenetic markerDNA sequencing

MeSH terms

AnimalsBase SequenceDNAEcologyFemaleGene AmplificationGenetic MarkersGenetic TechniquesMaleMolecular BiologyMolecular Sequence DataPaternityPolymorphism, GeneticMolecular Probe TechniquesDNA Fingerprinting

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Genetic variation among cat fish (Mystus cavasius) population assessed by randomly amplified polymorphic (RAPD) markers from Assam, India