articleTop 1% cited
Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli
Gene · 1991 · Vol. 100 · pp. 195–199
Rong Fu Wang(University of Georgia)Sidney R. Kushner✉
Bacteriophages and microbial interactionsBacterial Genetics and BiotechnologyRNA and protein synthesis mechanismsBiologyMolecular biologyPlasmidT7 RNA polymeraseGeneticsIn vitro recombinationGeneDNA polymerase IIPromoterCloning vector
MeSH terms
Ampicillin ResistanceBase SequenceCloning, MolecularColiphagesEscherichia coliGenetic MarkersGenetic VectorsMolecular Sequence DataPlasmidsRestriction MappingOligonucleotide ProbesGene ExpressionPolymerase Chain Reaction
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References
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors
Gene · 1985 · 15,158 citations
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Science · 1988 · 17,121 citations
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