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The use of DAPI for identifying and counting aquatic microflora1
Limnology and Oceanography · 1980 · Vol. 25(5) · pp. 943–948
Karen G. Porter✉(University of Georgia)Yvette S. Feig(University of Georgia)
Abstract
A highly specific and sensitive fluorescing DNA stain, 4′6‐diamidino‐2‐phenylindole (DAPI) was compared with acridine orange (AO) for counting aquatic microflora. Use of DAPI improved visualization and counting of <1‐ µ m bacteria and blue‐green algae in seston‐rich samples and extended sample storage to at least 24 weeks.
Microbial Community Ecology and PhysiologyMolecular Biology Techniques and ApplicationsIdentification and Quantification in FoodDAPIAcridine orangeAlgaeBiologyChemistryChromatographyStainingBotany
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