article
Improved DNA extraction from ancient bones using silica-based spin columns
American Journal of Physical Anthropology · 1998 · Vol. 105(4) · pp. 539–543
Dongya Yang(McMaster University)Barry Eng(McMaster University)John S. Waye(McMaster University)J. Christopher Dudar(McMaster University)Shelley R. Saunders✉(McMaster University)
Abstract
We describe a simple method for extracting polymerase chain reaction-amplifiable DNA from ancient bones without the use of organic solvents. Bone powders are digested with proteinase K, and the DNA is purified directly using silica-based spin columns (QIAquick3, QIAGEN). The efficiency of this protocol is demonstrated using human bone samples ranging in age from 15 to 5,000 years old.
Forensic and Genetic ResearchArchaeology and ancient environmental studiesIdentification and Quantification in FoodDNADNA extractionExtraction (chemistry)Polymerase chain reactionChromatographyAncient DNAHuman boneChemistryMaterials scienceMolecular biology
MeSH terms
AnimalsBone and BonesDNAFossilsHumansSilicon DioxideSpecimen HandlingPolymerase Chain Reaction
Citations
623
FWCI
1.81
field-weighted impact
References
8
Percentile
85%
vs. same field & year
Citations per year
References
Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia
Science · 1985 · 8,998 citations
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