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A Highly Efficient Escherichia coli-Based Chromosome Engineering System Adapted for Recombinogenic Targeting and Subcloning of BAC DNA

Genomics · 2001 · Vol. 73(1) · pp. 56–65

E-Chiang Lee(National Cancer Institute)Daiguan Yu(Frederick National Laboratory for Cancer Research)Jason Velasco(National Cancer Institute)Lino Tessarollo(Cancer Genetics (United States))Deborah A. Swing(National Cancer Institute)Donald L. Court(Frederick National Laboratory for Cancer Research)Nancy A. Jenkins(National Cancer Institute)Neal G. Copeland✉(National Cancer Institute)

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CRISPR and Genetic Engineering Animal Genetics and Reproduction Chromosomal and Genetic VariationsBacterial artificial chromosomeBiologyProphageRecombineeringSubcloningGeneticsgenomic DNAIn vitro recombinationGenomic libraryFLP-FRT recombination

MeSH terms

AnimalsCloning, MolecularDefective VirusesDNA, BacterialDNA, RecombinantEscherichia coliGenes, BacterialGenetic EngineeringMice, TransgenicOligodeoxyribonucleotidesBacteriophage lambdaPlasmidsRecombination, GeneticTransformation, BacterialGenes, Reporter

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