reviewTop 1% cited

Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations

Free Radical Biology and Medicine · 2011 · Vol. 52(1) · pp. 1–6

Balaraman Kalyanaraman✉(Medical College of Wisconsin)Victor Darley‐Usmar(University of Alabama at Birmingham)Kelvin J.A. Davies(University of Southern California)Phyllis A. Dennery(Children's Hospital of Philadelphia)Henry Jay Forman(University of Southern California)Matthew B. Grisham(Louisiana State University Health Sciences Center Shreveport)Giovanni E. Mann(King's College London)Kevin Moore(University College London)L. Jackson Roberts(Vanderbilt University)Harry Ischiropoulos(Children's Hospital of Philadelphia)

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Molecular Sensors and Ion Detection Redox biology and oxidative stress Analytical Chemistry and SensorsFluorescenceNitrogenReactive oxygen speciesOxygenReactive nitrogen speciesNanotechnologyBiological systemChemistryEnvironmental chemistryMaterials science

MeSH terms

Fluorescent DyesHydrogen PeroxideOxidation-ReductionSpectrometry, FluorescenceStaining and LabelingSuperoxidesReactive Oxygen SpeciesReactive Nitrogen SpeciesPeroxynitrous Acid

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The challenges of using fluorescent probes to detect and quantify specific reactive oxygen species in living cells