articleTop 1% cited
A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis
Electrophoresis · 2006 · Vol. 27(13) · pp. 2782–2786
Wei Wang✉(University of Siena)Rita Vignani(University of Siena)Monica Scali(University of Siena)M. Cresti(University of Siena)
Abstract
A simple and universally applicable protocol for extracting high-quality proteins from recalcitrant plant tissues is described. We have used the protocol with no modification, for a wide range of leaves and fruits. In all cases, this protocol allows to obtain good electrophoretic separation of proteins. As the protocol is rapid, universal, and compatible with silver staining, it could be used for routine protein extraction from recalcitrant plant tissues for proteomic analysis.
Proteins in Food SystemsAdvanced Proteomics Techniques and ApplicationsSoybean genetics and cultivationProtocol (science)Extraction (chemistry)ChromatographyComputational biologyProtein purificationChemistryBiologyBiochemistryMedicinePathology
MeSH terms
Electrophoresis, Polyacrylamide GelPlant ProteinsPlantsElectrophoresis, Gel, Two-DimensionalSilver StainingProteomics
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References
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