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A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody

European Journal of Immunology · 1979 · Vol. 9(3) · pp. 205–210

Andrew J. McMichael✉(University of Oxford)Jennifer R. Pilch(University of Oxford)John W. Fabre(University of Oxford)David Y. Mason(University of Oxford)Giovani Galfré(University of Oxford)César Milstein(University of Oxford)

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Abstract

Spleen cells from a BALB/c mouse that had been immunized with human thymocytes were fused with the myeloma line P3-NS 1/1 Ag 4.1. One of the resulting hybrid clones (NA 1/34) secreted an antibody that was highly specific for human thymocytes. Eighty-five % of thymocytes expressed the antigen designated HTA1. There were an estimated 15 x 10(4) molecules of HTA 1 per cell, and it is therefore a major surface molecule. The expression of this antigen on thymocytes appears to be reciprocal to HLA, as recognized by another monoclonal antibody W6/32. Immunoprecipitated material from [125I]-labeled thymocyte membranes was analyzed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate which disclosed a single component of 45,000 molecular weight.

Monoclonal and Polyclonal Antibodies Research T-cell and B-cell Immunology Glycosylation and Glycoproteins ResearchThymocyteBiologyMolecular biologyMonoclonal antibodyAntigenImmunoprecipitationAntibodyPolyacrylamide gel electrophoresisGel electrophoresisSodium dodecyl sulfate

MeSH terms

AnimalsAntibodiesAntibody SpecificityAntigensBinding SitesClone CellsElectrophoresis, Polyacrylamide GelFemaleHumansHybrid CellsHybridization, GeneticImmunoenzyme TechniquesMice, Inbred BALB CMyeloma ProteinsChemical Precipitation

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