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Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation

Journal of Immunological Methods · 2003 · Vol. 281(1-2) · pp. 65–78
Michael R. BettsJason M. BrenchleyDavid A. PriceStephen C. De RosaDaniel C. DouekMario RoedererRichard A. Koup
T-cell and B-cell ImmunologyImmune Cell Function and InteractionImmunotherapy and Immune ResponsesDegranulationCytotoxic T cellBiologyCD8Molecular biologyCytolysisCell biologyAntigenIntracellularPerforin

MeSH terms

Cytotoxicity, ImmunologicEnterotoxinsFlow CytometryHumansInterferon-gammaMembrane GlycoproteinsPlatelet Membrane GlycoproteinsSensitivity and SpecificityHistocompatibility Antigens Class ICell DegranulationAntigens, CDCD8-Positive T-LymphocytesLysosomal Membrane ProteinsLysosomal-Associated Membrane Protein 1Pore Forming Cytotoxic Proteins
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