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Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation
Journal of Immunological Methods · 2003 · Vol. 281(1-2) · pp. 65–78
Michael R. Betts✉(National Institutes of Health)Jason M. Brenchley(National Institute of Allergy and Infectious Diseases)David A. Price(National Institutes of Health)Stephen C. De Rosa(National Institutes of Health)Daniel C. Douek(National Institutes of Health)Mario Roederer(National Institutes of Health)Richard A. Koup(National Institute of Allergy and Infectious Diseases)
T-cell and B-cell ImmunologyImmune Cell Function and InteractionImmunotherapy and Immune ResponsesDegranulationCytotoxic T cellBiologyCD8Molecular biologyCytolysisCell biologyAntigenIntracellularPerforin
MeSH terms
Cytotoxicity, ImmunologicEnterotoxinsFlow CytometryHumansInterferon-gammaMembrane GlycoproteinsPlatelet Membrane GlycoproteinsSensitivity and SpecificityHistocompatibility Antigens Class ICell DegranulationAntigens, CDCD8-Positive T-LymphocytesLysosomal Membrane ProteinsLysosomal-Associated Membrane Protein 1Pore Forming Cytotoxic Proteins
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