articleTop 10% cited

New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites

Gene · 1988 · Vol. 74(2) · pp. 527–534

R. Daniel Gietz✉(National Institute of Environmental Health Sciences)Akio Sugino(National Institute of Environmental Health Sciences)

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CRISPR and Genetic Engineering Bacterial Genetics and Biotechnology Fungal and yeast genetics researchBiologyShuttle vectorSubcloningpUC19Restriction enzymeRestriction sitePlasmidMultiple cloning siteURA3Genetics

MeSH terms

AllelesDNA, RecombinantEscherichia coliGenes, FungalGenetic VectorsGenotypeMutationOligodeoxyribonucleotidesPlasmidsSaccharomyces cerevisiaeTransformation, GeneticRestriction Mapping

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2,899

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References

Transformation of intact yeast cells treated with alkali cations

Journal of Bacteriology · 1983 · 7,672 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Gene · 1985 · 15,158 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

Proceedings of the National Academy of Sciences · 1985 · 7,319 citations

Sterile host yeasts (SHY): A eukaryotic system of biological containment for recombinant DNA experiments

Gene · 1979 · 961 citations

Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 gene

Gene · 1979 · 1,073 citations

Studies on transformation of Escherichia coli with plasmids

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A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

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New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites

Gene · 1988 · 2,899 citations

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