article Open AccessTop 1% cited
Isolation of disaturated phosphatidylcholine with osmium tetroxide
Journal of Lipid Research · 1976 · Vol. 17(3) · pp. 281–284
Robert J. Mason✉(University of California San Francisco Medical Center)Jean Nellenbogen(University of California San Francisco Medical Center)Judith A. Clements(University of California San Francisco Medical Center)
Abstract
A simple, rapid, inexpensive method for isolating disaturated phosphatidylcholine from adult rat lung has been devised. Total lipids are reacted with osmium tetroxide dissolved in carbon tetrachloride, and the disaturated phosphatidylcholine is isolated on a column of neutral alumina. More than 99% of the fatty acids in the phosphatidylcholine fraction are saturated and 94% of this material migrates as phosphatidylcholine on subsequent thin-layer chromatography.
Fatty Acid Research and HealthBiochemical Analysis and Sensing TechniquesLipid metabolism and biosynthesisOsmium tetroxideIsolation (microbiology)ChemistryOsmiumChromatographyBiologyMicrobiologyBiochemistryPhysics
MeSH terms
AnimalsChemistryLipidsLungMercuryOsmiumPhosphatidylcholinesRatsChemical Phenomena
Citations
681
FWCI
14.17
field-weighted impact
References
25
Percentile
100%
vs. same field & year
Citations per year
Cited by
Plug the lung until it grows (PLUG): A new method to treat congenital diaphragmatic hernia in utero
Journal of Pediatric Surgery · 1994 · 314 citations
References
Phosphorus Assay in Column Chromatography
Journal of Biological Chemistry · 1959 · 12,851 citations
A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Journal of Biological Chemistry · 1957 · 64,580 citations
Surface active materials from dog lung. II. Composition and physiological correlations
American Journal of Physiology-Legacy Content · 1972 · 517 citations
Citation Network
How this paper connects to the literature. Drag to explore, click any node to open that paper.