article Open AccessTop 1% cited

Murine Sarcoma and Leukemia Viruses: Assay Using Clonal Lines of Contact-Inhibited Mouse Cells

Journal of Virology · 1969 · Vol. 4(5) · pp. 549–553

John Jainchill✉(National Cancer Institute)Stuart A. Aaronson(National Cancer Institute)George J. Todaro(National Cancer Institute)

View via DOI Full-text PDF

Abstract

A continuous cell line of highly contact-inhibited cells (NIH/3T3) has been developed from NIH Swiss mouse embryo cultures. Its growth properties are similar to those of 3T3 and BALB/3T3. Although 3T3 is relatively insensitive to focus formation by murine sarcoma viruses, cloned lines of both NIH/3T3 and BALB/3T3 have been isolated that are highly sensitive to sarcoma virus focus formation and leukemia virus growth. The sensitivity and specificity are comparable to those found with primary embryo cells. MSV-transformed lines of NIH/3T3 have been obtained.

Virus-based gene therapy research Animal Virus Infections Studies RNA Interference and Gene Delivery3T3 cellsBiologyCell cultureMurine leukemia virusVirologyLeukemiaContact inhibitionVirusEmbryoMolecular biology

MeSH terms

AnimalsCell LineCell Transformation, NeoplasticClone CellsContact InhibitionEmbryo, MammalianFibroblastsLeukemia Virus, MurineGammaretrovirusCulture TechniquesMice

Citations

823

FWCI

15.04

field-weighted impact

References

Percentile

99%

vs. same field & year

Citations per year

Cited by

Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family