A novel approach to depression management: antidepressant efficacy of Fermented Dacus Carota (Carrot) Kanji

Abstract

Objective: Our research will investigate the potential of anti-depressant effect of fermented Dacus carota (Carrrot) Kanji by using of various behavioral models like Forced Swim Test (FST), Tail Suspension Test (TST), We will also investigate brain neurotransmitter levels, including serotonin, and dopamine. Materials and Methods: Preparation of Carrot Kanji - The fermented carrot kanji was prepared using Daucus carota (carrot), water, salt, and mustard seeds The mixture was transferred into a clean, airtight container to maintain the integrity of the fermentation process. Experimental Animals: In this study, a total of 30 animals (adult male mice 25-30g; 6 animals per group) were used to assess the effects of Kanji and an antidepressant. These animals were housed in standard laboratory conditions with a 12-hour light/dark cycle, and they were provided with food and water ad libitum. Results and Discussion: The results of the behavioral and biochemical analyses demonstrated that Kanji, particularly at higher doses, exhibited antidepressant-like and anxiolytic-like effects, similar to the widely used antidepressant Fluoxetine. The Forced Swim Test (FST) and Tail Suspension Test (TST) both revealed significant reductions in immobility and increases in active behaviors with the higher doses of Kanji, suggesting its potential as a natural alternative for treating depressive-like disorders. Summary and Conclusion: The results from this study suggest that Kanji, particularly at higher doses, exhibits significant antidepressant-like and anxiolytic-like effects, comparable to the widely used antidepressant Fluoxetine. The behavioral tests such as the Forced Swim Test (FST), Tail Suspension Test (TST), demonstrated that Kanji effectively reduced immobility time and promoted active coping behaviors, suggesting potential therapeutic benefits for treating depressive-like and anxiety-like symptoms.