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Mechanistic insights into polyphenol oxidase activity and black spot onset in Aquacultured white leg shrimp (Litopenaeus vannamei)
International Journal of Advanced Biochemistry Research · 2025 · Vol. 9(12) · pp. 1223–1230
Abstract
The black spot formation in Litopenaeus vannamei kept in chilled storage (2 °C) was examined for 14 days. The crustacean enzyme polyphenol oxidase (PPO) was partially purified through 70% ammonium sulfate precipitation, followed by dialysis. The PPO activities in various parts of L. vannamei were assessed using 3,4-dihydroxy phenylalanine (L-DOPA) as the substrate. The melanosis scores considerably (p
Polyamine Metabolism and ApplicationsAquaculture Nutrition and GrowthInvertebrate Immune Response MechanismsShrimpPolyphenol oxidaseLitopenaeusCatechol oxidaseBlack spotPolyphenolPrawnEnzyme
Funding
- Indian Council of Agricultural Research
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