In vitro management of Pectobacterium carotovorum subsp. carotovorum the causal organism of carrot soft rot disease using chemicals and antibiotics

Abstract

The effectiveness of four chemical substances and five antibiotics was assessed in vitro against Pectobacterium carotovorum subsp. carotovorum, the pathogen responsible for carrot soft rot disease. Pectobacterium carotovorum subsp. carotovorum was isolated from diseased carrot samples using the serial dilution technique, and various biochemical and pathogenicity tests were conducted to confirm the bacterial species. chemicals viz., copper oxychloride, copper hydroxide, nano zinc and nano copper at 0.005, 0.01, 0.02 and 0.03 per cent and antibiotics viz., tagmycin, bacterinashak, plantamycin, kasugamycin, validamycin at 50, 100, 250, 300 and 500 ppm were tested against the pathogen by well diffusion method. Data were recorded at 96 hour after incubation and results showed that among the chemicals copper oxy chloride exhibited greater inhibition zone (9.68 mm) followed by nano zinc (5.60 mm) and nano copper (4.78 mm). Among the different antibiotics tagmycin (14.13 mm) showed maximum inhibition zone followed by bacterinashak (10.92 mm). Copper oxychloride followed by nano zinc and tagmycin followed by bacterinashak, were found to be the most effective chemical and antibiotic treatments, respectively, for the in vitro management of Pectobacterium carotovorum subsp. carotovorum causing soft rot in carrot.