In-vitro organogenesis studies in Aglaonema var. red valentine (Aglaonema communtatum), super white (Aglaonema spp.), red anjamani (Aglaonema spp.)

Abstract

An efficient in vitro regeneration protocol was standardized for Aglaonema varieties Red valentine, Super white and Red anjamani using axillary bud explants. Sequential sterilization with Savlon, Tween-20, Carbendazim, Cefotaxime, ethanol, sodium hypochlorite and HgCl₂ achieved up to 88.66% aseptic cultures. Shoot initiation was optimal on MS medium with 3 mg/L BAP for Red valentine and 5 mg/L BAP for Super white and Red anjamani. Combined BAP + Kinetin gave maximum shoot multiplication (2.44 - 3.10 shoots/explant). Rooting was highest with IBA + NAA (94-96.43%). Cocopeat proved best for primary hardening and a 1:1 mix of red soil and cocopeat ensured >95% survival in secondary hardening. The protocol enables large-scale, disease-free, true-to-type Aglaonema production.