In vitro organogenesis techniques in golden bamboo (Phyllostachys aurea)

Abstract

The present investigation aimed to develop a standardized in vitro organogenesis protocol for the production of disease and virus free plantlets of Golden Bamboo (Phyllostachys aurea). The study was conducted in a Completely Randomized Design at the Plant Biotechnology Centre, Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, Dist. Ratnagiri (M.S.) during the academic years 2023-2025.Among the various surface sterilization treatments of axillary nodal explants. The sequential treatment comprising Tween-20 (5.0%), Alcohol (70%), Carbendazim (0.2%), Streptomycin (0.2%), Ethanol (60%), NaOCl (3.0%), H2O2 (0.2%) and HgCl2 (0.1%) recorded the highest aseptic culture establishment (43.66%) and survival rate (87.33%). The nodal explant with 3 cm in length and 3 mm in diameter exhibited the highest bud sprouting response achieved 85.66% with 87.33% survival rate. Murashige and Skoog (MS) medium supplemented with 5.0 mg/l BAP and additives facilitated 90.66% shoot initiation with shoot induction, producing an average of 6.66 shoots per explant with a mean shoot length of 3.0 cm. The highest shoot multiplication rate (4.66) was obtained on MS medium fortified with 0.5 mg/l BAP with 6.0 cm shoot length. The rooting was most effective (88.66%) on MS medium containing 1.0 mg/l IBA and 1.5 mg/l NAA, resulting in an average of 9.66 roots per explant within 12 days. The plantlets acclimatized in a 1:1:1 potting mixture of red soil: cocopeat: vermicompost achieved a plantlet survival rate of 80.33%.