Optimisation of culture conditions for cellulase production by Arthrobacter arilaitensis

Abstract

Microbial Cellulase production is strongly influenced by factors such as temperature, pH, and incubation period. The present study evaluated the optimal culture conditions for cellulase synthesis by Arthrobacter arilaitensis isolated from horse dung. The strain exhibited maximum enzyme activity at 37 °C (0.0952±0.0057 U/mL), pH 7.0 (0.0644±0.0079 U/mL) and 72 hours of incubation (0.0921±0.0048 U/mL). Enzyme activity decreased significantly beyond these conditions. These findings confirm that A. arilaitensis is a mesophilic, neutral-pH-adapted cellulase producer, suitable for applications in ruminant nutrition and lignocellulosic biomass utilization.