A comparative study of rhizosphere and endophytic fungi from Ruta graveolens and its antibacterial study

Abstract

The study of Endophytic fungi in the medicinal plants showed quite a variation. In total five fungi Trichoderma viridae, Chalaropsis radicicola, Penicillium pseudostromaticum, Colletotrichum sp. and Fusarium solani were isolated from rhizosphere while four fungi Bispora sp., Chalaropsis radicicola, Penicillium pseudostromaticum and Fusarium solani were isolated as endophytes from the plant Ruta graveolens. Two types of rhizosphere fungi Trichoderma viridae and Colletotrichum sp. were found to be 33.33% of the population of total fungi isolated while the only endophyte fungi Bispora sp.was 16.66%. Three isolates Chalaropsis radicicola Penicillum pseudostromaticum and Fusarium solani were common both as rhizospheric and endophytic being 50% of the total population of the isolates. The fungal isolate Chalaropsis radicicola from the plant Ruta gravolens,showed inhibition of gram negative bacteria K. pneumoniae. The dominant endophytic fungi Fusarium solani showed inhibition of both of the gram negative bacteria K. pneumoniae and E. coli. The dominant endophyte Penicillium sp., showed inhibition of only gram positive bacteria S. aureus. The endophyte Bispora sp., did not show any antibacterial activity.