Investigation on blood biochemicals and enzymes profile of Jaffarabadi buffalo calves

Abstract

An attempt was made to investigate the profile of blood biochemicals and enzymes in Jaffarabadi buffalo calves. The study involved 24 calves and was conducted at the Cattle Breeding Farm, Kamdhenu University, Junagadh, following approval from the Institutional Animal Ethics Committee. Iso-managerial condition was ensured. Pelleted concentrate was offered to meet protein requirements feeding standards. A total 3 blood collections from each calf, at 8th, 91st and 182nd day of age were made. The blood biochemicals viz., glucose, cholesterol, triglycerides, total protein, albumin and globulin, blood urea nitrogen (BUN), bilirubin, creatinine, insulin like growth factor (IGF-1): and the enzymes like lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and asparate amino-transferase (AST) and antioxidants like super-oxide dismutase (SOD) and catalase activity were measured using standard kit method. The alterations in the biochemicals and enzymes up to 6 months of age, early (day-8 to 91) and late (day-91 to 182) post-natal phases, were ascertained. Overall mean values of glucose, cholesterol, triglycerides, total protein and albumin were 74.56±1.83 mg/dL, 77.00±1.24 mg/dL, 29.37±0.52 mg/dL and 7.15±0.03 g/dL and 3.34±0.07, respectively. The levels of glucose, cholesterol, triglycerides, and albumin marginally, non-significantly declined by -8.75, -7.81, -14.90, -10.38 respectively; however, these were within the normal physiological range. Total protein and globulin levels increased by 1.90% and 11.84%, respectively. Serum urea nitrogen, bilirubin and creatinine averaged 13.84±0.20,0.17±0.01 and 1.17±0.03 mg/dL, the levels remained almost static and in normal range. The overall mean of the IGF-1 was 45.05±2.65 ng/ml. A highly significant (p