Establishment of gout induction model through sodium bicarbonate intoxication in broilers

Abstract

The gout condition is regularly occurred in commercial poultry farming in spite of various treatment options. Hence, it’s highly essential to formulate a suitable remedy for gout irrespective of etiology and to study the antigout activity it’s a prerequisite to standardize a suitable gout induction model in broiler chicken. In this context, the present study was conducted in forty broiler chicks of 10 days old divided into five groups with triplicate as per experimental design. The gout was induced by dissolving toxic dose of sodium bicarbonate (SBC) powder in drinking water at different rates (5 g/ L. 10 g/L, 15 g/L and 20 g/L) on 11th day of age. The blood samples were collected on day 10 (before induction), 15 and 18 for serum uric acid and creatinine estimation. The clinical manifestations, mortality due to gout induction were noted and gross and histopathology studies were conducted. The data collected on biochemical parameters were analyzed statistically by One-way ANOVA using SPSS software (version 20). The gout signs like watery droppings, dullness, depression and unthriftiness and mortality were occurred within three days in 20 g/L group and higher serum uric acid (24.13±0.97 mg/dL), creatinine (0.73±0.03 mg/dL) values were noted on day 15. Further, gross pathology and histopathology findings confirmed development of gout in broiler chicken. Hence, it was concluded that administration of SBC @ 20 g/L could be a reliable model to study the antigout activity of any medicinal preparations in broiler chicken.