Detection, identification and characterization of pathogenic bacteria from wound infection

Abstract

This study was aimed to isolate & identify the pathogenic bacteria from collected wound samples by selective culture medium, standard biochemical tests and 16s rRNA sequencing. Purulent materials from different wound infection were collected aseptically with the aid of sterile swab sticks from 100 out of 25 patients of Namakkal district. Seven types of bacterial species were isolated and identified by selective culture medium and standard biochemical tests from the collected wound samples. 16S rRNA gene PCR and subsequent were applied to 4 bacterial wound isolates after enrichment by automated culture. Each wound samples showed one or more bacterial isolate, totally 78.9% of samples exhibited 43 isolates. The most predominant bacterial isolate was Staphylococcus aureus (36%) followed by Pseudomonas aeruginosa (18%), Klebsiella pneumoniae (14%) and Escherichia coli (11%) from 100 different wound samples were identified and characterized 16S ribosomal RNA gene sequencing. Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomoas aeruginosa isolates were characterized by sequencing process. The results were confirmed the identity of the isolates. All the clinical isolates were 100% similarity with respective species. 16S rRNA gene sequencing allowed bacterial identification that was reproducible and more accurate then that obtained by phenotypic testing. 16S rRNA gene sequencing analysis would be helpful in timely and correct identification of pathogens.