In vitro evaluation of different fungicides and bio agents against jackfruit leaf spot caused by Colletotrichum gloeosporioides

Abstract

The leaf spot disease caused by Colletotrichum gloeosporioides is most common and serious disease of jackfruit, which significantly decreased the market value of fruits. In present research work, Six fungicides Carbendazim 12% + Mancozeb 63% WP, Carbendazim 50% WP, Copper oxycloride 50% WP, Thiophanate methyl 70% WP, Hexaconazole 5% SC, Tebuconazole 50% + Trifloxystrobin 25% WG concentrations at 0.2%, 0.1%, 0.25%, 0.1%, 0.05% and 0.05% respectively and seven bio agents viz., Trichoderma viride, Trichoderma harzianum, Trichoderma longibrachiatun, Trichoderma koningii, Pseudomonas fluorescens, Bacillus subtillis and Aspergillus niger were evaluated in vitro against Colletotrichum gloeosporioides causing leaf spot of jackfruit, using “Poisoned Food Technique” for fungicide evaluation and “Dual culture technique” for bio agents evaluation with three replications in Completely Randomized Design. Among the fungicides evaluated Carbendazim 12% + Mancozeb 63% WP @ 0.2% and Carbendazim 50% WP @ 0.1% showed 100 percent inhibition of mycelial growth of Colletotrichum gloeosporioides. While Copper oxycloride 50% WP @ 0.25% was least effective which showed 61.65% inhibition. Among the used Bioagents, Trichoderma longibrachiatum was most effective in inhibiting the mycelial growth of test fungus with 77.98% (19.81 mm). Bacillus subtilis was least effective in controlling the mycelial growth of Colletotrichum gloeosporioides (40.16%).