The analytical method for the simultaneous estimation of naproxen and domperidone will be developed by rp-HPLC method by optimizing the chromatographic conditions

Abstract

Domperidone and naproxen in tablet dosage form can be analyzed simultaneously using a straightforward, fast, and selective reversed phase High Performance Liquid Chromatographic (RP-HPLC) method that has been developed and validated. The CTO-10ASVP column oven, SPD-20A UV detector, SIL-20A auto-sampler, and two LC-20 AT pumps made up the chromatographic system. A Shim-Pack Cix column (250 mm x 4.6 mm, 5 µm) was used as the stationary phase for the chromatographic separation of drugs. The mobile phase consisted of a 30:70 (v/v) ratio of methanol to phosphate buffer (pH adjusted to 3.00 with sodium hydroxide) at a flow rate of 1.0 ml/min, with UV detection at 280 nm. 3.17 was the retention time. 5.42 minutes for naproxen, and minutes for domperidone. Domperidone and naproxen peaks were found to be well separated by the technique, which was shown to be selective (resolution 10.72). The suggested approach is accurate with a 99.5% recovery for domperidone and a 99.39% recovery for naproxen, linear (r=0.999 for both drugs), and precise (%RSD < 1%). The commercial product&#39;s potency was determined using this procedure, and it was found to be within a certain range. Domperidone and naproxen in tablet dosage form can be analyzed using this method.