Determination of Quercetin by UV spectroscopy in feed premix

Abstract

A simple, accurate, precise and economic UV spectroscopic method for the determination of quercetin.The estimation of quercetin by spectroscopic with maximum absorption at max 373 nm using ethanol.Beer-Lambert's law is obeyed in the concentration range of 5-15g/ml and is described by the regression equation y=0.0823x + 0.0109 with a regression coefficient (r2) = 0.999 (N=5).LOD and LOQ are found to be 1.46 and 4.43 g/ml, respectively.The percentage recovery of quercetin was found to be 97%.The% RSD for interday and intraday precision was 1.769 and 0.938 respectively showed excellent% RSD which is less than 2%.The developed method was validated in terms accuracy, precision, linearity and robustness.The statistically validated results indicate that the proposed method has good sensitivity, accuracy and precision.The method is simple and economic as compare to chromatographic methods.The developed method was successfully applied for the determination of Quercetin in feed premix.There is no spectroscopic method is available for the estimation of quercetin in Feed premix formulation.The aim of this research is to reduce analysis time for the determination of quercetin in Feed premix formulation by UV spectroscopy.