Evaluation of phenotypic characteristics and confirmatory tests of isolates from Cajanus cajan

Abstract

The goal of the current study is to separate and screen soil samples from the Rajgarh district in the state of Madhya Pradesh for the morphological and biochemical traits of several isolates of Cajanus cajan. After three to five days of incubation on YEMA plates, the isolates generated from Cajanus cajan formed white colonies with a diameter of one to three millimeters. The colonies were round, raised, and produced a significant amount of mucus. Five of the 32 isolates that were examined showed gram-positive staining, whereas 27 showed gram-negative staining. Most of the isolates exhibited rod-shaped cells, except for 4 isolates that were coccus-shaped. On YEMA-Cr plates, the isolates generally showed white colonies, although 5 isolates absorbed the Congo red dye, resulting in red colonies. The ketolactose test indicated that 6 of the isolates could produce the ketolactose enzyme.