Study of plasmid-mediated Amp-C beta lactamases activity among klebsiella pneumoniae isolated from patients of Tanta university hospitals

Abstract

Background: The primary enzymatic mechanism for antibiotic resistance in Klebsiella pneumoniae (KP) is Amp-C beta lactamases. Aim: Finding the Amp-C-Beta-Lactamase gene that causes antibiotic resistance in Klebsiella pneumonia isolated from patients at Tanta University hospitals was the goal of the current study. Methods and subjects: B-lactamase at 100 Amp-C The synthesis of Amp-C was examined in Klebsiella pneumoniae. The majority of Amp-C positive strains were successfully detected by three phenotypic Amp-C confirmation assays (Amp-C E test, the disc approximation test, and Amp C EDTA disc). Multiplex PCR was then used to molecularly detect plasmid-mediated Amp-C in order to detect the CMY-1, CMY-2, and FOX-1 genes Results: Among 100 MDR Klebsiella pneumoniae isolates, positive isolates for Amp-C by phenotypic tests were detected in 34 isolates (34%). Then by application of multiplex PCR on the positive isolates for phenotypic tests there were (26) isolates out of (34) isolates harbored Amp-C genes including CMY-1which was detected in (65.3%) (17/26), followed by CMY-2 was detected in (50.0%) (13/26), finally FOX-1 genes were detected in (34.6%) (9/26), so CMY-1 gene was the predominant type in all isolates. Conclusion: It was determined that: The Tanta University Hospital has a growing number of isolates of Klebsiella pneumoniae strains that produce Amp-C. Therefore, a trustworthy epidemiological study of their spread in hospitals would need molecular identification of the genes expressing Amp-C.