Assessment of insulin receptor substrate-1 (G972R) SNP and correlated with total antioxidant capacity in T2DM patients

Abstract

Background and Aim: The prevalence and incidence of Type 2 Diabetes Mellitus (T2DM) have significantly increased globally in the past few years, around two to three times each. A lower age of onset is caused by a number of risk factors, such as female sex, maternal diabetes or gestational diabetes, ethnicity, family history, and extreme obesity. The aim of the present research to assess levels of Malondialdehyde (MDA) and Total Antioxidant Capacity (TAOC) as a markers of the oxidative stress in Iraqi patients diagnosed with Type 2 Diabetes Mellitus (T2DM) and correlate with G972R SNP of ISR-1 gene. Methods: The Babylon province was the site of this research in the period from September 1, 2023, to March 30, 2024. There were 90 subjects in all for the study: Relatives of hospital patients and hospital staff without type 2 diabetes were used to enroll 50 patients with the disease and 40 healthy controls. The two research cohorts were matched for age. The following parameters were ascertained by analyzing blood samples from both groups: Glycated Hemoglobin (HbA1c), Total Antioxidant Capacity (TAOC), and malondialdehyde (MDA) as a measure of oxidative stress. PCR-RFLP used for genotyping analysis of G972R SNP. Results: Compared to the control group, the T2DM group's mean serum TAOC was considerably lower (32.21±3.12 U/ml vs. 52.22±4.76 U/ml). This suggests that there is a very substantial difference between the groups. Furthermore, TAOC and HbA1c showed statistically significant inverse relationships in the T2DM group. The RR genotype creates 212, 31 bp bands (Which did not appear on our gel), the GG genotype produces just 243 bp bands, and the GR genotype produces 243, 212, 31 bp bands, according to the results of the G972R SNP analysis using the restriction enzyme MvaI. Conclusion: This research shown strong correlation between increase oxidative stress and lowering of TAOC with the incidence of T2DM in Iraqi population through the significant alteration in G972R SNP of ISR-1 gene.