Evaluation of the synergistic effect of herbal drugs Embelia ribes, Cinnamomum verum and Zingiber officinale by in vitro anti-inflammatory studies

Abstract

Herbal drugs have a great significance in the field of therapeutics. In this work we have selected three herbal drugs i.e.; Embelia ribes, Cinnamomum verum and Zingiber officinale which have proven anti-inflammatory property individually, now our study focuses on the synergistic effect of the above three drugs by in-vitro Anti-inflammatory studies. Preliminary phytochemical screening has been performed primarily and followed by the evaluation for the proposed activity. The main action of anti-inflammatory agents is the inhibition of Cyclooxygenase enzymes which are responsible for the conversion of Arachidonic acid to prostaglandins. Since human red blood cell (HRBC) membranes are similar to these lysosomal membrane components, the prevention of hypotonicity induced HRBC membrane lysis was taken as a measure in estimating the anti-inflammatory property of extracts of Embelia ribes, Cinnamomum verum and Zingiber officinale. Thus, Human red blood cell membrane stabilization (HRBC method) has been used as a method in estimating the anti-inflammatory property. The present study aimed to authenticate that traditional information by in vitro anti-inflammatory screening.