Why optimizing thresholds in forensic genetics matters

Abstract

In forensic genetics, the accurate analysis and interpretation of DNA trace is paramount. To ensure reliability, scientists employ cut-off and threshold values at various stages of DNA analysis, including quantitative real-time PCR (qPCR) and capillary electrophoresis of short tandem repeat (STR) amplicons. These thresholds help distinguish true genetic signals from background noise, ensuring the integrity of forensic trace evidence.