Studies on in-vitro shoot and root induction in turmeric cv. PDKV Waigaon

Abstract

The present investigation entitled “Studies on in-vitro shoot and root induction in turmeric cv. PDKV Waigaon” was conducted at Centre of Excellence, Plant Biotechnology, Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, (M.S.) during the academic year 2021-2022 with the aim to standardized the protocol for in-vitro shoot and root induction in turmeric with (CRD) Completely Randomized Design having 5 treatments and 4 replications of in-vitro shoot induction and 3 treatments and 7 replications of in-vitro root induction. In this study the surface sterilized rhizome buds were cultured on MS medium supplemented with different combinations of BAP and NAA, the highest number of shoots and number of leaves per shoot was recorded in treatment MS media + 2 mg/l BAP + 1 mg/l NAA, whereas, minimum number of days for shoot initiation and days for shoot proliferation after shoot initiation were also recorded in the same treatment combination. In in-vitro root induction half strength of MS medium was used with different combinations of IBA, the highest number of roots per plant and the lowest days for root initiation were observed in treatment combination ½MS media + 1 mg/l IBA. In the present study above combinations of growth regulators were found suitable for the successful propagation of turmeric with the help of tissue culture technique.