Chemo-profiling of aqueous extract of Nauclea latifolia using GC-XX

Abstract

Momordica dioica Roxb. ex Willd. is a perennial, dioecious plant (2n = 28) from the Cucurbitaceae family. Traditional methods of propagation, such as using seeds, stem cuttings, and rhizomatous/tuberous roots, are insufficient for large-scale cultivation as a vegetable crop. This study presents an enhanced and efficient micropropagation technique for wild female M. dioica using nodal explants. Shoot proliferation was achieved by subculturing in vitro raised shoots on Murashige and Skoog (MS) medium supplemented with concentrations of 6-benzylaminopurine (BAP) 2 mg l-1 in combination with L- Glutamic acid (LGA) 2 mg l-1. In vitro produced shoots then transformed on half strength MS media containing different concentrations of auxins IBA and IAA form which IBA 2mg l-1 produce roots in 12.88 days with root length 9.45 cm and 4.22 number of roots per shoots.