CRISPR-Cas9-mediated genome editing in silkworm (Bombyx mori L.)

Abstract

The CRISPR-Cas9 system has revolutionized genome editing, with significant applications in B. mori (silkworm), a key organism in sericulture. This review highlights the use of CRISPR-Cas9 in enhancing silk production, improving disease resistance, and advancing silkworm biology. Researchers have used CRISPR to increase silk yield by manipulating genes related to growth regulation and to create stronger silk by integrating spider silk genes. Additionally, CRISPR has enabled the development of antiviral-resistant silkworms, reducing the impact of pathogens like B. mori nucleopolyhedrovirus (BmNPV), a major threat to sericulture. Beyond industrial applications, CRISPR has facilitated deeper exploration into silkworm genetics, including studies on gene function and developmental processes. These advancements not only offer direct benefits to silk production but also position CRISPR-Cas9 as a powerful tool for sustainable and resilient sericulture. This review underscores CRISPR’s transformative role in improving silkworm strains and shaping the future of sericulture to meet global demands efficiently and sustainably.