Biochemical effects of ingested crude oil contaminated Mangifera indica stem bark aqueous and ethanol extracts in wistar albino rats

Abstract

The impact of crude oil spills on food safety is increasingly concerning due to associated health issues. In Nigeria, rural areas, which are major sites of crude oil exploration, produce most of the food crops. This study aimed to evaluate the biochemical effects of aqueous and ethanolic extracts of crude oil-contaminated Mangifera indica stem bark on adult female Wistar albino rats. One hundred and twenty-five rats were divided into three groups: control, aqueous, and ethanol extract groups, with each extract group further divided into crude oil-free and contaminated sub-groups. The sub-groups were administered doses of 85 mg/kg, 170 mg/kg, 255 mg/kg, 340 mg/kg, 425 mg/kg, and 510 mg/kg. After 12 weeks of treatment, the rats were euthanized, and blood samples were analyzed for renal function markers such as creatinine and urea levels. Results indicated that crude oil-contaminated extracts significantly (p0.05), with the ethanolic extract showing the most significant (p