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Molecular characterization of a Porcine Group A rotavirus of VP6 gene of by Reverse Transcriptase–Polymerase Chain Reaction

International Journal of Veterinary Sciences and Animal Husbandry · 2024 · Vol. 9(5) · pp. 381–383

UM Tumlam✉KM BorateDM MuglikarMS PawadeS.A. JadhavCS Mote

Abstract

The present study aimed to detect Rotavirus in the feces of diarrheic piglets using a VP6 gene-based RT-PCR assay. 15 fecal samples from piglets aged 0-6 months were analyzed for the presence of group A Rotavirus. Results showed that 10 out of the 15 samples (66.6%) tested positive for the Rotavirus VP6 gene, indicating a significant prevalence of group A Rotavirus in this population. It was observed that piglets less than one-month-old exhibited a higher rate of Rotavirus detection compared to older piglets. The findings suggest that the RT-PCR assay is a highly sensitive and specific method for the rapid detection of group A Rotavirus in fecal samples from piglets.

Viral gastroenteritis research and epidemiology Animal Virus Infections Studies Virus-based gene therapy researchReverse transcriptasePolymerase chain reactionGeneRotavirusPolymeraseMolecular biologyVirologyNested polymerase chain reactionReverse transcription polymerase chain reactionChemistry

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